DNA methylation and mRNA expression of imprinted genes in blastocysts derived from an improved in vitro maturation method for oocytes from small antral follicles in polycystic ovary syndrome patients.

Does imprinted DNA methylation or imprinted gene expression differ between human blastocysts from conventional ovarian stimulation (COS) and an optimized two-step IVM method (CAPA-IVM) in age-matched polycystic ovary syndrome (PCOS) patients?No significant differences in imprinted DNA methylation and gene expression were detected between COS and CAPA-IVM blastocysts.

Animal models have revealed alterations in DNA methylation maintenance at imprinted germline differentially methylated regions (gDMRs) after use of ARTs. This effect increases as more ART interventions are applied to oocytes or embryos. IVM is a minimal-stimulation ART with reduced hormone-related side effects and risks for patients.

Mouse Monoclonal Anti-Chlamydia LPS IgG
AB-13010 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD80 IgG
AB-13310 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD11a IgG
AB-13510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD4 IgG
AB-14510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD8 IgG
AB-14810 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD4 IgG
AB-16510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD5 IgG
AB-17110 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD3 IgG
AB-17510 100 ug
EUR 347

Mouse Monoclonal Anti-SARS Spike IgG
AB-17910 50 ug
EUR 469

Mouse monoclonal Anti-HA-tag peptide IgG
AB-10110 100 ug
EUR 469

Mouse Monoclonal Anti-Human Interleukin-2 IgG
AB-10210 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-2 IgG
AB-10510 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-3 IgG
AB-10610 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-4 IgG
AB-10710 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-4 IgG
AB-10810 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-6 IgG
AB-10910 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-7 IgG
AB-11010 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-8 IgG
AB-11110 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-10 IgG
AB-11210 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-10 IgG
AB-11310 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-12p40 IgG
AB-11410 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-15 IgG
AB-11510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD8-FITC IgG
AB-13110 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD4-FITC IgG
AB-13210 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD8-biotinylated IgG
AB-13410 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD11b-FITC IgG
AB-13610 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD3-FITC IgG
AB-13710 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD3-biotinylated IgG
AB-13810 100 ug
EUR 347

Rat Monoclonal Anti-Mouse B220 (CD45R) IgG
AB-13910 100 ug
EUR 347

Mouse monoclonal Anti-Human CD3 IgG, unlabled
AB-14410 100 ug
EUR 469

Mouse Monoclonal Anti- HIV-1 p24 IgG
AB-15210 100 ug
EUR 347

Mouse Monoclonal Anti-HIV-2 gp39 IgG
AB-15310 100 ug
EUR 347

Mouse Monoclonal Anti-SARS Spike Protein IgG
AB-15710 20 ug
EUR 408

Mouse Monoclonal Anti-HIV-1 gp41 IgG
AB-15910 100 ug
EUR 347

Mouse Monoclonal Anti-HIV-2 gp39 IgG
AB-16110 1 ml
EUR 347

Mouse monoclonal Anti-HIV-2 p17 IgG
AB-16120 100 ug
EUR 469

Rat Monoclonal Anti-Mouse IFN-gamma IgG
AB-16610 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD4-biotinylated IgG
AB-16710 100 ug
EUR 347

Mouse Monoclonal Anti-SARS Nucleocapsid protein IgG
AB-17810 50 ug
EUR 469

Mouse Monoclonal Anti-SARS Nucleocapsid Protein IgG
AB-18010 50 ug
EUR 469

Mouse Monoclonal Anti-Myelin Oligodendrocyte Glycoprotein IgG
AB-19910 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-12p75 IgG
AB-20910 100 ug
EUR 347

Mouse Monoclonal Anti-Human c-Myc IgG
AB-21110 100 ug
EUR 347

ELISA kit for Mouse ACV-AB (Activin AB)  Kit
E-EL-M2660 1 plate of 96 wells
EUR 534
  • Gentaur's ACV-AB ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ACV-AB. Standards or samples are added to the micro ELISA plate wells and combined wit
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Mouse ACV-AB (Activin AB)  Kit in samples from Serum, Plasma, Cell supernatant

ACV-AB ELISA Kit| Mouse Activin AB ELISA Kit
EF012990 96 Tests
EUR 689

Rat Monoclonal Anti-Mouse Interleukin-2 receptor IgG
AB-10310 100 ug
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Mouse Monoclonal Anti-Human Interleukin-2 receptor IgG
AB-10410 100 ug
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Hamster Mono Anti-Mouse CTLA-4 (CD152) IgG
AB-11610 100 ug
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Mouse Monoclonal Anti-Human Eotaxin-1 (CCL11) IgG
AB-12610 100 ug
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Rat Monoclonal Anti-Mouse CD90 Thy-1.1 IgG
AB-14010 100 ug
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AB-14110 100 ug
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AB-14210 100 ug
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Mouse Monoclonal Anti-Hepatitis C Virus (HCV) NS3 IgG
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AB-20410 100 ug
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Mouse Monoclonal Anti-Human FAS (CD95) Activating antibody IgG
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AB-20710 100 ug
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AB-16310 1 ml
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AB-15110 100 ug
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Mouse Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit
CN-02423M1 96T
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Mouse Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit
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Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit
GA-E0148MS-48T 48T
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Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit
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Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit
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Mouse BB-AB ELISA Kit
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ELISA kit for Mouse PDGF-AB (Platelet Derived Growth Factor AB)
E-EL-M0631 1 plate of 96 wells
EUR 534
  • Gentaur's PDGF-AB ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGF-AB. Standards or samples are added to the micro ELISA plate wells and combined w
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Mouse PDGF-AB (Platelet Derived Growth Factor AB) in samples from Serum, Plasma, Cell supernatant

Mouse Activin AB (ACVAB) ELISA Kit
abx254671-96tests 96 tests
EUR 754
  • Shipped within 5-12 working days.

ELISA kit for Mouse PDGF-AB
EK5212 96 tests
EUR 519
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse PDGF-AB in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse PDGF-AB PicoKine ELISA Kit
EK0486 96 wells
EUR 456
Description: For quantitative detection of mouse PDGF-AB in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Mouse Activin AB (ACVAB) ELISA Kit
CEA158Mu-10x96wellstestplate 10x96-wells test plate
EUR 4862.4
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Activin AB (ACVAB) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit
CEA158Mu-1x48wellstestplate 1x48-wells test plate
EUR 488.08
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Activin AB (ACVAB) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit
CEA158Mu-1x96wellstestplate 1x96-wells test plate
EUR 654.4
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Activin AB (ACVAB) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

CAPA-IVM is an improved IVM system that includes a pre-maturation step (CAPA), followed by an IVM step, both in the presence of physiological compounds that promote oocyte developmental capacity.For DNA methylation analysis 20 CAPA-IVM blastocysts were compared to 12 COS blastocysts. For RNA-Seq analysis a separate set of 15 CAPA-IVM blastocysts were compared to 5 COS blastocysts.

COS embryos originated from 12 patients with PCOS (according to Rotterdam criteria) who underwent conventional ovarian stimulation.

For CAPA-IVM 23 women were treated for 3-5 days with highly purified hMG (HP-hMG) and no hCG trigger was given before oocyte retrieval. Oocytes were first cultured in pre-maturation medium (CAPA for 24 h containing C-type natriuretic peptide), followed by an IVM step (30 h) in medium containing FSH and Amphiregulin. After ICSI, Day 5 or 6 embryos in both groups were vitrified and used for post-bisulphite adaptor tagging (PBAT) DNA methylation analysis or RNA-seq gene expression analysis of individual embryos.

Data from specific genes and gDMRs were extracted from the PABT and RNA-seq datasets.CAPA-IVM blastocysts showed similar rates of methylation and gene expression at gDMRs compared to COS embryos. In addition, expression of major epigenetic regulators was similar between the groups.The embryos from the COS group were generated in a range of culture media. The CAPA-IVM embryos were all generated using the same sperm donor.

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