Does imprinted DNA methylation or imprinted gene expression differ between human blastocysts from conventional ovarian stimulation (COS) and an optimized two-step IVM method (CAPA-IVM) in age-matched polycystic ovary syndrome (PCOS) patients?No significant differences in imprinted DNA methylation and gene expression were detected between COS and CAPA-IVM blastocysts.
Animal models have revealed alterations in DNA methylation maintenance at imprinted germline differentially methylated regions (gDMRs) after use of ARTs. This effect increases as more ART interventions are applied to oocytes or embryos. IVM is a minimal-stimulation ART with reduced hormone-related side effects and risks for patients.
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3750-1HP-10 |
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3750-1HP-2 |
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56625 |
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100 ug |
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56626 |
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56627 |
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EUR 651 |
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EUR 351.75 |
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EUR 687.75 |
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3376-1H-20 |
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EUR 651 |
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EUR 351.75 |
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3432-1A-20 |
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EUR 651 |
ELISA Flex: Mouse IL-10 (ALP) |
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3432-1A-6 |
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3432-1H-20 |
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EUR 651 |
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3432-1H-6 |
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EUR 351.75 |
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2237 |
Genovis AB |
100ug |
EUR 445 |
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3311-2A |
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EUR 609 |
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3311-2H |
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2 plates |
EUR 535.5 |
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3311-4HPW-10 |
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10 plates |
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3311-4HPW-2 |
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EUR 535.5 |
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3361-2A |
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3391-2A |
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EUR 609 |
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3391-2H |
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EUR 609 |
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10 plates |
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EUR 609 |
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EUR 651 |
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EUR 351.75 |
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3521-1H-20 |
Mabtech AB |
for 20 plates |
EUR 651 |
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3521-1H-6 |
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EUR 351.75 |
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3376-2A |
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EUR 609 |
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3376-2H |
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EUR 609 |
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3376-4APW-10 |
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3432-2A |
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EUR 609 |
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3432-4APW-10 |
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3432-4HPW-10 |
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3317-1A-20 |
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EUR 351.75 |
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3317-1H-20 |
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EUR 651 |
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3317-1H-6 |
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EUR 351.75 |
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3521-2A |
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EUR 609 |
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EUR 609 |
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3521-4APW-10 |
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EUR 2420.25 |
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2 plates |
EUR 535.5 |
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3521-4HPW-10 |
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10 plates |
EUR 2420.25 |
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FSP-42B-10 |
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10 plates |
EUR 2541 |
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EUR 561.75 |
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|
3321-1A-20 |
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for 20 plates |
EUR 651 |
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|
3321-1A-6 |
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for 6 plates |
EUR 351.75 |
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|
3321-1H-20 |
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for 20 plates |
EUR 651 |
ELISA Flex: Mouse IFN-gamma (HRP) |
|
3321-1H-6 |
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for 6 plates |
EUR 351.75 |
CAPA-IVM is an improved IVM system that includes a pre-maturation step (CAPA), followed by an IVM step, both in the presence of physiological compounds that promote oocyte developmental capacity.For DNA methylation analysis 20 CAPA-IVM blastocysts were compared to 12 COS blastocysts. For RNA-Seq analysis a separate set of 15 CAPA-IVM blastocysts were compared to 5 COS blastocysts.
COS embryos originated from 12 patients with PCOS (according to Rotterdam criteria) who underwent conventional ovarian stimulation.
For CAPA-IVM 23 women were treated for 3-5 days with highly purified hMG (HP-hMG) and no hCG trigger was given before oocyte retrieval. Oocytes were first cultured in pre-maturation medium (CAPA for 24 h containing C-type natriuretic peptide), followed by an IVM step (30 h) in medium containing FSH and Amphiregulin. After ICSI, Day 5 or 6 embryos in both groups were vitrified and used for post-bisulphite adaptor tagging (PBAT) DNA methylation analysis or RNA-seq gene expression analysis of individual embryos.
Data from specific genes and gDMRs were extracted from the PABT and RNA-seq datasets.CAPA-IVM blastocysts showed similar rates of methylation and gene expression at gDMRs compared to COS embryos. In addition, expression of major epigenetic regulators was similar between the groups.The embryos from the COS group were generated in a range of culture media. The CAPA-IVM embryos were all generated using the same sperm donor.
