Human PCR Endothelial lipase (EL) Elisa Kit

Goals

To evaluate and compare the performance of five commercial ELISA assays (EDI, AnshLabs, Dia. Pro, NovaTec and Lionex) to detect anti-SARS-CoV-2 IgG.

Methods

Seventy negative control samples (collected before the COVID-19 pandemic) and samples from 101 patients with RT-PCR-and-Elisa-kits-el confirmed SARS-CoV-2 (collected at different times from symptom onset: ≤7, 8–14 and >14 days) were used to compare the sensitivity, specificity, concordance, and positive and negative predictive values ​​of each RT-PCR assay. An assessment of agreement between the five trials was also performed. Cross-reactivity with other HCoV viruses, non-HCoV respiratory viruses, non-respiratory viruses, and nuclear antigens was investigated.

Product name

Endothelial Lipase (EL), ELISA kit

Full product name

Human Endothelial Lipase (EL) ELISA Kit

Product gene name

Elisa kit

For research use only

For research use only. It should not be used in diagnostic procedures.

OMIM: 603684

Species reactivity: Human

Specificity: No cross-reaction with other factors.

Samples: Cell culture supernatant, human plasma or serum

Test type: Quantitative sandwich

Detection range: 5000pg/ml-78pg/ml

Sensitivity: Up to 20pg/mL

Intra-assay precision: <= 8%

Inter-assay precision: <= 12%

Preparation and Storage: Store all reagents at 2-8 degrees C.

ISO certification

Manufactured in an ISO 9001:2015 certified laboratory.

Product note

Information selected from the online data sheet is extracted from bioinformatics databases, sometimes resulting in the ambiguous or non-relevant product information. It is the customer’s responsibility to review, verify and evaluate the information to ensure it matches their requirements prior to purchasing the kit. Our ELISA Kit assays are dynamic research tools and may be updated and improved from time to time.

If the format of this assay is important to you, please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current trial details. We cannot guarantee that the sample manual posted online is the most up-to-date manual, it is intended to serve as an example only. Consult the instructions for use provided with the assay kit for precise details.

Other notes

Occasionally, small volumes of ELISA kit vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly spin the vial in a tabletop centrifuge to dislodge any liquid in the vial cap. Certain products may require dry ice shipping and an additional dry ice fee may apply.

Search terms for buying EL

MBS265611 is a ready-to-use microwell strip plate ELISA (Enzyme-Linked Immunosorbent Assay) Kit for testing the presence of the target analytes of the Endothelial Lipase (EL) ELISA Kit in biological samples. The concentration gradients of the kit standards or positive controls generate a theoretical detection range of the kit in biological research samples containing EL.

The ELISA analytical biochemical technique of the MBS265611 kit is based on EL antibody-EL antigen (immunosorbent) interactions and an HRP colourimetric detection system to detect EL antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant EL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays that evaluated reproducibility identified intra-assay CV (%) and inter-assay CV (%).

Product related information for ELISA kits

Assay Principle: This experiment uses the double-sandwich ELISA technique and the provided ELISA kit is typical. The pre-coated antibody is a human EL monoclonal antibody and the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated label antibodies are added to the wells of the ELISA plate and washed with PBS or TBS. The avidin-peroxidase conjugates are then added to the ELISA wells in order; Use TMB Substrate for staining after the reagent is thoroughly washed with PBS or TBS. TMB turns blue under catalytic peroxidase and finally turns yellow under the action of acid. Colour depth and test factors in samples are positively correlated.