Does imprinted DNA methylation or imprinted gene expression differ between human blastocysts from conventional ovarian stimulation (COS) and an optimized two-step IVM method (CAPA-IVM) in age-matched polycystic ovary syndrome (PCOS) patients?No significant differences in imprinted DNA methylation and gene expression were detected between COS and CAPA-IVM blastocysts.

Animal models have revealed alterations in DNA methylation maintenance at imprinted germline differentially methylated regions (gDMRs) after use of ARTs. This effect increases as more ART interventions are applied to oocytes or embryos. IVM is a minimal-stimulation ART with reduced hormone-related side effects and risks for patients.

Goat anti-Rat IgG (H&L), F(ab)'2 fragment, HRP conjugated, min,reactivity to human, mouse IgG

AS10-1145 0,5 mg
EUR 246

Goat anti-Human IgG Fc, F(ab)'2 fragment, HRP conjugated, min, cross-reactivity bovine/mouse/rabbit serum

AS10-817 0,5 mg
EUR 233

Goat anti-Human IgM (µ chain), F(ab)'2 Fragment, min, cross-reactivity to IgG from human or mouse

AS16-3371 1,0 mg
EUR 238

Goat anti-Human IgG Fc, F(ab)'2 fragment, Biotin conjugated, min, cross-reactivity bovine/mouse/rabbit serum

AS10-814 0,5 mg
EUR 246

Goat anti-Rat IgG (H&L), F(ab)'2 fragment, FITC conjugated, min, cross-reactivity to human, mouse IgG

AS10-1234 0,5 mg
EUR 246

Goat anti-Rat IgG (H&L), F(ab)'2 fragment, Biotin conjugated, min, cross-reactivity to human, mouse IgG

AS10-1023 0,5 mg
EUR 246

Goat anti-Rat IgG (H&L), F(ab)'2 fragment, TRITC conjugated, min, cross-reactivity to human, mouse IgG

AS10-1088 0,5 mg
EUR 246

Goat anti-Human IgG (H&L), F(ab)'2 fragment, HRP conjugated, min, cross-reactivity bovine/mouse/rabbit serum

AS10-785 0,5 mg
EUR 233

Goat anti-Human IgG Fc, F(ab)'2 fragment, ALP conjugated, min, cross-reactivity to bovine/mouse/rabbit serum

AS10-813 0,5 mg
EUR 246

Goat anti-Human IgG Fc, F(ab)'2 fragment, TRITC conjugated, min, cross-reactivity to bovine,mouse,rabbit serum

AS10-1001 0,5 mg
EUR 246

Goat anti-Human IgG Fc, F(ab)'2 fragment, FITC conjugated, min, cross-reactivity to bovine,mouse,rabbit serum

AS10-1099 0,5 mg
EUR 246

Goat anti-Human IgG (H&L), F(ab)'2 fragment, Biotin conjugated, min, cross-reactivity bovine/mouse/rabbit serum

AS10-782 0,5 mg
EUR 233

Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, FITC conjugated, min, cross-reactivity to human, mouse,Rat IgG

AS10-1058 0,5 mg
EUR 246

Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, HRP conjugated, min, cross-reactivity to human, mouse,Rat IgG

AS10-1065 0,5 mg
EUR 246

Goat anti-Human IgG (H&L), F(ab)'2 fragment, ALP conjugated, min, cross-reactivity to bovine/mouse/rabbit serum

AS10-781 0,5 mg
EUR 206

Goat anti-Human IgG (H&L), F(ab)'2 fragment, FITC conjugated, min, cross-reactivity to bovine,mouse,rabbit serum

AS10-1015 0,5 mg
EUR 233

Goat anti-Human IgG (H&L), F(ab)'2 fragment, TRITC conjugated, min, cross-reactivity to bovine,mouse,rabbit serum

AS10-1157 0,5 mg
EUR 233

Goat anti-Rat IgG (H&L), F(ab)'2 fragment, Affinity purified, Unconjugated, min, cross-reactivity to human, mouse IgG

AS10-1215 1 mg
EUR 233

Goat anti-Rat IgG (H&L), F(ab)'2 fragment, DyLight® 488 conjugated, min, cross-reactivity to human, mouse lgG

AS10-1217 0,5 mg
EUR 259

Goat anti-Rat IgG (H&L), F(ab)'2 fragment, DyLight® 550 conjugated, min, cross-reactivity to human, mouse lgG

AS12-1980 0,5 mg
EUR 246

Goat anti-Human IgG Fc, F(ab)'2 Fragment, min, cross-reactivity to Human IgA/IgM, Bovine, Horse, Mouse, Rabbit Serum

AS16-3350 0,5 mg
EUR 216

Goat anti-Human IgG Fc, F(ab)'2 fragment, Affinity purified, Unconjugated, min, cross-reactivity to bovine/mouse/rabbit serum

AS10-812 1 mg
EUR 233

Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, FITC conjugated, min, cross-reactivity to bovin, human, mouse IgG/serum

AS10-1042 0,5 mg
EUR 233

Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated, min, cross-reactivity to bovine,human, mouse IgG/serum

AS10-1146 0,5 mg
EUR 233

Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, TRITC conjugated, min, cross-reactivity to bovine,human, mouse IgG/serum

AS10-1059 0,5 mg
EUR 233

Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, Biotin conjugated, min, cross-reactivity to bovine,human, mouse IgG/serum

AS10-1153 0,5 mg
EUR 233

Rabbit anti-Goat IgG (H&L), F(ab)'2 fragment, Affinity purified, Unconjugated, min, cross-reactivity to human, mouse,Rat IgG

AS10-1077 1 mg
EUR 246

Goat anti-Human IgG (H&L), F(ab)'2 fragment, Affinity purified, Unconjugated, min, cross-reactivity to bovine/mouse/rabbit serum

AS10-780 1 mg
EUR 233

Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, Affinity purified, Unconjugated, min, cross-reactivity to bovine, human, mouse IgG/serum

AS10-982 1 mg
EUR 190

Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, ALP (alkaline phosphatase) conjugated, min, cross-reactivity to bovine,human, mouse IgG/serum

AS10-1017 0,5 mg
EUR 233

Goat anti-Mouse IgG Fc, F(ab)´2 Fragment

AS16-3485 1,0 mg
EUR 206

Donkey anti-Rabbit IgG (H&L), F(ab)'2 Fragment, min, cross-reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat or Sheep

AS16-3522 0,5 mg
EUR 213

Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, DyLight® 550 conjugated,min, cross-reactivity to bovine, human, or Mouse lgG or serum proteins

AS12-1968 0,5 mg
EUR 206

Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, DyLight® 594 conjugated, min, cross-reactivity to bovine, human, or Mouse lgG or serum proteins

AS12-2083 0,5 mg
EUR 206

Goat anti-Rabbit IgG (H&L) -F(ab)'2 fragment, DyLight® 650 conjugated, min, cross-reactivity to bovine, human, or Mouse lgG or serum proteins

AS12-2337 0,5 mg
EUR 220

Mouse Ig fraction

AS10-911 10 mg
EUR 139

Donkey anti-Mouse IgG (H&L), F(ab)'2 Fragment

AS16-3437 1,0 mg
EUR 224

ELISA Pro: Mouse apoE

3752-1HP-1 1 plate
EUR 515.2
Description: Mouse

ELISA Pro: Mouse apoE

3752-1HP-10 10 plates
EUR 2945.6
Description: Mouse

ELISA Pro: Mouse apoE

3752-1HP-2 2 plates
EUR 744.8
Description: Mouse

ELISA Pro: Mouse apoA1

3750-1HP-1 1 plate
EUR 515.2
Description: Mouse

ELISA Pro: Mouse apoA1

3750-1HP-10 10 plates
EUR 2945.6
Description: Mouse

ELISA Pro: Mouse apoA1

3750-1HP-2 2 plates
EUR 744.8
Description: Mouse

Anti-Mouse Alpha Synuclein Mouse Monoclonal Antibody

56625 100 ug
EUR 357

Anti-Mouse Alpha Synuclein Mouse Monoclonal Antibody

56627 100 ug
EUR 357

GST-tag (mouse, monoclonal)

AS21-4677 50 µg
EUR 155

V5 (mouse antibody (monoclonal)

AS21-4676 50 µg
EUR 155

ELISA Pro: Mouse IL-5

3391-1HP-1 1 plate
EUR 537.6
Description: Mouse

ELISA Pro: Mouse IL-5

3391-1HP-10 10 plates
EUR 3068.8
Description: Mouse

ELISA Pro: Mouse IL-5

3391-1HP-2 2 plates
EUR 778.4
Description: Mouse

ELISA Pro: Mouse IL-2

3441-1HP-1 1 plate
EUR 537.6
Description: Mouse

ELISA Pro: Mouse IL-2

3441-1HP-10 10 plates
EUR 3068.8
Description: Mouse

ELISA Pro: Mouse IL-2

3441-1HP-2 2 plates
EUR 778.4
Description: Mouse

ELISA Pro: Mouse IL-4

3311-1HP-1 1 plate
EUR 537.6
Description: Mouse

ELISA Pro: Mouse IL-4

3311-1HP-10 10 plates
EUR 3068.8
Description: Mouse

ELISA Pro: Mouse IL-4

3311-1HP-2 2 plates
EUR 778.4
Description: Mouse

ELISA Pro: Mouse TNF-α

3511-1HP-1 1 plate
EUR 537.6
Description: Mouse

ELISA Pro: Mouse TNF-α

3511-1HP-10 10 plates
EUR 3068.8
Description: Mouse

ELISA Pro: Mouse TNF-α

3511-1HP-2 2 plates
EUR 778.4
Description: Mouse

ELISA Pro: Mouse IFN-γ

3321-1HP-1 1 plate
EUR 537.6
Description: Mouse

ELISA Pro: Mouse IFN-γ

3321-1HP-10 10 plates
EUR 3068.8
Description: Mouse

ELISA Pro: Mouse IFN-γ

3321-1HP-2 2 plates
EUR 778.4
Description: Mouse

ELISA Flex: Mouse IgE (ALP)

3815-1A-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IgE (HRP)

3815-1H-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IgG (ALP)

3825-1AD-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IgG (HRP)

3825-1HD-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IgA (ALP)

3865-1AD-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IgM (ALP)

3885-1A-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IgM (HRP)

3885-1H-6 for 6 plates
EUR 397.6
Description: Mouse

Mouse normal serum (2 ml)

AS10-1543 2 ml
EUR 167

Mouse normal serum (5 ml)

AS10-1544 5 ml
EUR 139

Mouse purified IgG (10 mg)

AS10-912 10 mg
EUR 206

ELISA Flex: Mouse IgEa (ALP)

3816-1A-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IgEa (HRP)

3816-1H-6 for 6 plates
EUR 397.6
Description: Mouse

ELISpot Flex: Mouse IgE (ALP)

3815-2A for 4 plates
EUR 565.6
Description: Mouse

ELISpot Flex: Mouse IgE (HRP)

3815-2H for 4 plates
EUR 565.6
Description: Mouse

ELISpot Flex: Mouse IgG (ALP)

3825-2A for 4 plates
EUR 565.6
Description: Mouse

ELISpot Flex: Mouse IgG (HRP)

3825-2H for 4 plates
EUR 565.6
Description: Mouse

ELISpot Flex: Mouse IgA (ALP)

3865-2A for 4 plates
EUR 565.6
Description: Mouse

ELISpot Flex: Mouse IgA (HRP)

3865-2H for 4 plates
EUR 565.6
Description: Mouse

ELISpot Flex: Mouse IgM (ALP)

3885-2A for 4 plates
EUR 565.6
Description: Mouse

ELISpot Flex: Mouse IgM (HRP)

3885-2H for 4 plates
EUR 565.6
Description: Mouse

ELISpot Flex: Mouse IgEa (ALP)

3816-2A for 4 plates
EUR 565.6
Description: Mouse

ELISpot Flex: Mouse IgEa (HRP)

3816-2H for 4 plates
EUR 565.6
Description: Mouse

ELISpot Flex: Mouse IgG1 (ALP)

3821-2A for 4 plates
EUR 688.8
Description: Mouse

ELISpot Flex: Mouse IgG1 (HRP)

3821-2H for 4 plates
EUR 688.8
Description: Mouse

ELISpot Flex: Mouse IgG3 (ALP)

3826-2A for 4 plates
EUR 688.8
Description: Mouse

ELISpot Flex: Mouse IgG3 (HRP)

3826-2H for 4 plates
EUR 688.8
Description: Mouse

ELISpot Flex: Mouse IgG2a (ALP)

3822-2A for 4 plates
EUR 688.8
Description: Mouse

ELISpot Flex: Mouse IgG2a (HRP)

3822-2H for 4 plates
EUR 688.8
Description: Mouse

ELISpot Flex: Mouse IgG2b (ALP)

3823-2A for 4 plates
EUR 688.8
Description: Mouse

ELISpot Flex: Mouse IgG2b (HRP)

3823-2H for 4 plates
EUR 688.8
Description: Mouse

ELISpot Flex: Mouse IgG2c (ALP)

3824-2A for 4 plates
EUR 688.8
Description: Mouse

ELISpot Flex: Mouse IgG2c (HRP)

3824-2H for 4 plates
EUR 688.8
Description: Mouse

Agrisera IncuBlocker (mouse antibody)

AS16-3215 2 x 50 ml
EUR 130

FluoroSpot Flex: Mouse IgG/IgA

FS-49R50M-1 1 plate
EUR 487.2
Description: Mouse

FluoroSpot Flex: Mouse IgG/IgA

FS-49R50M-10 10 plates
EUR 4144
Description: Mouse

FluoroSpot Flex: Mouse IgM/IgG

FS-60G49R-1 1 plate
EUR 487.2
Description: Mouse

FluoroSpot Flex: Mouse IgM/IgG

FS-60G49R-10 10 plates
EUR 4144
Description: Mouse

FluoroSpot Flex: Mouse IgM/IgA

FS-60G50M-1 1 plate
EUR 487.2
Description: Mouse

FluoroSpot Flex: Mouse IgM/IgA

FS-60G50M-10 10 plates
EUR 4144
Description: Mouse

StimPack: Memory B cells, mouse

3661-1 100 µg + 0.5 µg
EUR 218.4

ELISA Pro: Mouse IL-12 p70

3456-1HP-1 1 plate
EUR 537.6
Description: Mouse

ELISA Pro: Mouse IL-12 p70

3456-1HP-10 10 plates
EUR 3068.8
Description: Mouse

ELISA Pro: Mouse IL-12 p70

3456-1HP-2 2 plates
EUR 778.4
Description: Mouse

ELISA Flex: Mouse IL-6 (ALP)

3361-1A-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-6 (ALP)

3361-1A-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-6 (HRP)

3361-1H-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-6 (HRP)

3361-1H-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-5 (ALP)

3391-1A-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-5 (ALP)

3391-1A-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-5 (HRP)

3391-1H-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-5 (HRP)

3391-1H-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-2 (ALP)

3441-1A-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-2 (ALP)

3441-1A-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-2 (HRP)

3441-1H-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-2 (HRP)

3441-1H-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-4 (ALP)

3311-1A-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-4 (ALP)

3311-1A-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-4 (HRP)

3311-1H-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-4 (HRP)

3311-1H-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse TNF-α (ALP)

3511-1A-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse TNF-α (ALP)

3511-1A-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse TNF-α (HRP)

3511-1H-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse TNF-α (HRP)

3511-1H-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-22 (ALP)

3376-1A-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-22 (ALP)

3376-1A-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-22 (HRP)

3376-1H-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-22 (HRP)

3376-1H-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-1α (ALP)

3417-1A-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-1α (ALP)

3417-1A-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-1α (HRP)

3417-1H-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-1α (HRP)

3417-1H-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-10 (ALP)

3432-1A-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-10 (ALP)

3432-1A-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-10 (HRP)

3432-1H-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-10 (HRP)

3432-1H-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-1β (ALP)

3317-1A-20 for 20 plates
EUR 733.6
Description: Mouse

ELISA Flex: Mouse IL-1β (ALP)

3317-1A-6 for 6 plates
EUR 397.6
Description: Mouse

ELISA Flex: Mouse IL-1β (HRP)

3317-1H-20 for 20 plates
EUR 733.6
Description: Mouse

CAPA-IVM is an improved IVM system that includes a pre-maturation step (CAPA), followed by an IVM step, both in the presence of physiological compounds that promote oocyte developmental capacity.For DNA methylation analysis 20 CAPA-IVM blastocysts were compared to 12 COS blastocysts. For RNA-Seq analysis a separate set of 15 CAPA-IVM blastocysts were compared to 5 COS blastocysts.

COS embryos originated from 12 patients with PCOS (according to Rotterdam criteria) who underwent conventional ovarian stimulation.

For CAPA-IVM 23 women were treated for 3-5 days with highly purified hMG (HP-hMG) and no hCG trigger was given before oocyte retrieval. Oocytes were first cultured in pre-maturation medium (CAPA for 24 h containing C-type natriuretic peptide), followed by an IVM step (30 h) in medium containing FSH and Amphiregulin. After ICSI, Day 5 or 6 embryos in both groups were vitrified and used for post-bisulphite adaptor tagging (PBAT) DNA methylation analysis or RNA-seq gene expression analysis of individual embryos.

Data from specific genes and gDMRs were extracted from the PABT and RNA-seq datasets.CAPA-IVM blastocysts showed similar rates of methylation and gene expression at gDMRs compared to COS embryos. In addition, expression of major epigenetic regulators was similar between the groups.The embryos from the COS group were generated in a range of culture media. The CAPA-IVM embryos were all generated using the same sperm donor.