Does imprinted DNA methylation or imprinted gene expression differ between human blastocysts from conventional ovarian stimulation (COS) and an optimized two-step IVM method (CAPA-IVM) in age-matched polycystic ovary syndrome (PCOS) patients?No significant differences in imprinted DNA methylation and gene expression were detected between COS and CAPA-IVM blastocysts.

Animal models have revealed alterations in DNA methylation maintenance at imprinted germline differentially methylated regions (gDMRs) after use of ARTs. This effect increases as more ART interventions are applied to oocytes or embryos. IVM is a minimal-stimulation ART with reduced hormone-related side effects and risks for patients.

Rat Monoclonal Anti-Mouse CD11a IgG

AB-13510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD4 IgG

AB-14510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD8 IgG

AB-14810 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD4 IgG

AB-16510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD5 IgG

AB-17110 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD3 IgG

AB-17510 100 ug
EUR 347

Mouse Monoclonal Anti-SARS Spike IgG

AB-17910 50 ug
EUR 469

ACV-AB ELISA Kit| Mouse Activin AB ELISA Kit

EF012990 96 Tests
EUR 689

ELISA kit for Mouse ACV-AB (Activin AB)  Kit

E-EL-M2660 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse ACV-AB (Activin AB)  Kit in samples from Serum, Plasma, Cell supernatant

Mouse monoclonal Anti-HA-tag peptide IgG

AB-10110 100 ug
EUR 469

Mouse Monoclonal Anti-Human Interleukin-2 IgG

AB-10210 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-2 IgG

AB-10510 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-3 IgG

AB-10610 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-4 IgG

AB-10710 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-4 IgG

AB-10810 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-6 IgG

AB-10910 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-7 IgG

AB-11010 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-8 IgG

AB-11110 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-10 IgG

AB-11210 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-10 IgG

AB-11310 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-12p40 IgG

AB-11410 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-15 IgG

AB-11510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD8-FITC IgG

AB-13110 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD4-FITC IgG

AB-13210 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD8-biotinylated IgG

AB-13410 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD11b-FITC IgG

AB-13610 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD3-FITC IgG

AB-13710 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD3-biotinylated IgG

AB-13810 100 ug
EUR 347

Rat Monoclonal Anti-Mouse B220 (CD45R) IgG

AB-13910 100 ug
EUR 347

Mouse monoclonal Anti-Human CD3 IgG, unlabled

AB-14410 100 ug
EUR 469

Mouse Monoclonal Anti- HIV-1 p24 IgG

AB-15210 100 ug
EUR 347

Mouse Monoclonal Anti-HIV-2 gp39 IgG

AB-15310 100 ug
EUR 347

Mouse Monoclonal Anti-SARS Spike Protein IgG

AB-15710 20 ug
EUR 408

Mouse Monoclonal Anti-HIV-1 gp41 IgG

AB-15910 100 ug
EUR 347

Mouse Monoclonal Anti-HIV-2 gp39 IgG

AB-16110 1 ml
EUR 347

Mouse monoclonal Anti-HIV-2 p17 IgG

AB-16120 100 ug
EUR 469

Rat Monoclonal Anti-Mouse IFN-gamma IgG

AB-16610 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD4-biotinylated IgG

AB-16710 100 ug
EUR 347

Mouse Monoclonal Anti-SARS Nucleocapsid protein IgG

AB-17810 50 ug
EUR 469

Mouse Monoclonal Anti-SARS Nucleocapsid Protein IgG

AB-18010 50 ug
EUR 469

Mouse Monoclonal Anti-Myelin Oligodendrocyte Glycoprotein IgG

AB-19910 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-12p75 IgG

AB-20910 100 ug
EUR 347

Mouse Monoclonal Anti-Human c-Myc IgG

AB-21110 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-2 receptor IgG

AB-10310 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-2 receptor IgG

AB-10410 100 ug
EUR 347

Hamster Mono Anti-Mouse CTLA-4 (CD152) IgG

AB-11610 100 ug
EUR 347

Mouse Monoclonal Anti-Human Eotaxin-1 (CCL11) IgG

AB-12610 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD90 Thy-1.1 IgG

AB-14010 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD90 Thy-1.2 IgG

AB-14110 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD90 Thy-1 IgG

AB-14210 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD1A (T6, LEU6) IgG

AB-20610 100 ug
EUR 347

Mouse Monoclonal Anti-Hepatitis C Virus (HCV) NS3 IgG

AB-15610 20 ug
EUR 408

Mouse Monoclonal Anti-Human FAS (CD95) Blocking antibody IgG

AB-20410 100 ug
EUR 347

Mouse Monoclonal Anti-Human FAS (CD95) Activating antibody IgG

AB-20510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD2 (T11, LFA-2) IgG

AB-20710 100 ug
EUR 347

Mouse Monoclonal Anti-Hepatitis B Surface Antigen A (HBsAg) IgG

AB-16310 1 ml
EUR 347

Mouse Thyroid Ab ELISA Kit

ELA-E0844m 96 Tests
EUR 865

Mouse PDGF-AB ELISA Kit

LF-EK50843 1×96T
EUR 648

Mouse BB-AB ELISA Kit

EMB0647 96Tests
EUR 521

Mouse BP180-Ab ELISA Kit

EMB0691 96Tests
EUR 521

Mouse CaM-ab ELISA Kit

EMC0688 96Tests
EUR 521

Mouse CYT Ab ELISA Kit

EMC0878 96Tests
EUR 521

Mouse DNP-AB ELISA Kit

EMD0256 96Tests
EUR 521

Mouse ENA AB ELISA Kit

EME0159 96Tests
EUR 521

Mouse ES-Ab ELISA Kit

EME0181 96Tests
EUR 521

Mouse GAD-Ab ELISA Kit

EMG0305 96Tests
EUR 521

Mouse GnRHR-Ab ELISA Kit

EMG0347 96Tests
EUR 521

Mouse IFAa-AB ELISA Kit

EMI0346 96Tests
EUR 521

Mouse MAG Ab ELISA Kit

EMM0224 96Tests
EUR 521

Mouse MBP-Ab ELISA Kit

EMM0248 96Tests
EUR 521

Mouse MC Ab ELISA Kit

EMM0249 96Tests
EUR 521

Mouse MP-Ab ELISA Kit

EMM0341 96Tests
EUR 521

Mouse NB-Ab ELISA Kit

EMN0081 96Tests
EUR 521

Mouse RNP-AB ELISA Kit

EMR0365 96Tests
EUR 521

Mouse TPO-AB ELISA Kit

EMT0538 96Tests
EUR 521

Mouse UU-Ab ELISA Kit

EMU0025 96Tests
EUR 521

Mouse Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit

CN-02423M1 96T
EUR 457

Mouse Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit

CN-02423M2 48T
EUR 306

Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit

GA-E0148MS-48T 48T
EUR 336

Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit

GA-E0148MS-96T 96T
EUR 534

Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit

QY-E21208 96T
EUR 361

Mouse Monoclonal Anti-Dengue Virus Type 2 envelop IgG, aff pure

AB-14310 100 ug
EUR 347

Mouse Monoclonal Anti-Hepatitis B Virus (HBsAg;AD & AY Antigens) IgG

AB-15010 100 ug
EUR 347

Mouse Monoclonal Anti-HIV-1 gp120 (PND, principal neutralizing domain) IgG

AB-15110 100 ug
EUR 347

ELISA kit for Mouse PDGF-AB (Platelet Derived Growth Factor AB)

E-EL-M0631 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse PDGF-AB (Platelet Derived Growth Factor AB) in samples from Serum, Plasma, Cell supernatant

Soyasaponin Ab

HY-N3026 5mg
EUR 282

AB-423

HY-112142 100mg
EUR 2058

AB-680

HY-125286 10mg
EUR 1772

AB-MECA

HY-19365 5mg
EUR 380

Activin AB

PR15011 5 ug
EUR 481

Mouse Activin AB (ACVAB) ELISA Kit

CEA158Mu-10x96wellstestplate 10x96-wells test plate
EUR 4862.4
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit

CEA158Mu-1x48wellstestplate 1x48-wells test plate
EUR 488.08
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit

CEA158Mu-1x96wellstestplate 1x96-wells test plate
EUR 654.4
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit

CEA158Mu-5x96wellstestplate 5x96-wells test plate
EUR 2644.8
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit

4-CEA158Mu
  • EUR 4913.00
  • EUR 2595.00
  • EUR 655.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Mouse Activin AB (ACVAB) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Anti-Q-Ab ELISA Kit

EMA0827 96Tests
EUR 521

Mouse BM-CSF Ab ELISA Kit

EMB0673 96Tests
EUR 521

Mouse HSV I Ab ELISA Kit

EMH0316 96Tests
EUR 521

Mouse IFN-α-Ab ELISA Kit

EMI0048 96Tests
EUR 521

Mouse γ IFN-Ab ELISA Kit

EMI0421 96Tests
EUR 521

Mouse LP Ab-lgA ELISA Kit

EML0259 96Tests
EUR 521

Mouse LP Ab-lgG ELISA Kit

EML0260 96Tests
EUR 521

Mouse LP Ab-lgM ELISA Kit

EML0261 96Tests
EUR 521

Mouse LTF-AB-lgG ELISA Kit

EML0302 96Tests
EUR 521

Mouse N-DNA-AB ELISA Kit

EMN0082 96Tests
EUR 521

Mouse PR3 ab-lgG ELISA Kit

EMP0717 96Tests
EUR 521

Mouse Scl-70 Ab ELISA Kit

EMS0249 96Tests
EUR 521

Mouse Activin AB (ACVAB) CLIA Kit

20-abx490029
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Activin AB (ACVAB) ELISA Kit

abx254671-96tests 96 tests
EUR 754

PDGF-AB ELISA Kit (Mouse) (OKBB00241)

OKBB00241 96 Tests
EUR 544
Description: Description of target: The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 3 pg/mL

ELISA kit for Mouse PDGF-AB

EK5212 96 tests
EUR 519
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse PDGF-AB in samples from serum, plasma, tissue homogenates and other biological fluids.

PDGF-AB/ Rat PDGF- AB ELISA Kit

ELA-E0112r 96 Tests
EUR 886

MBP-Ab/ Rat MBP- Ab ELISA Kit

ELA-E0170r 96 Tests
EUR 886

MC Ab/ Rat MC Ab ELISA Kit

ELA-E0390r 96 Tests
EUR 886

TPO-Ab/ Rat TPO- Ab ELISA Kit

ELA-E0442r 96 Tests
EUR 886

Thyroid Ab/ Rat Thyroid Ab ELISA Kit

ELA-E0844r 96 Tests
EUR 886

GNRHR-Ab/ Rat GNRHR- Ab ELISA Kit

ELA-E0962r 96 Tests
EUR 886

MAG Ab/ Rat MAG Ab ELISA Kit

ELA-E0995r 96 Tests
EUR 886

DNP-Ab/ Rat DNP- Ab ELISA Kit

ELA-E1122r 96 Tests
EUR 886

ENA-Ab/ Rat ENA- Ab ELISA Kit

ELA-E1124r 96 Tests
EUR 886

RNP-Ab/ Rat RNP- Ab ELISA Kit

ELA-E1129r 96 Tests
EUR 886

Mouse Meckelin (Mks3) Control/blocking peptide

AB-23268-P 100ug
EUR 164

Mouse dermatopontin (Dpt) Control/blocking peptide

AB-23026-P 100ug
EUR 164

Mouse pannexin 3 (Panx3) Control/blocking peptide

AB-23242-P 100ug
EUR 164

Mouse pannexin 3 (Panx3) Control/blocking peptide

AB-23243-P 100ug
EUR 164

Mouse pannexin 3 (Panx3) Control/blocking peptide

AB-23244-P 100ug
EUR 164

Mouse pannexin 1 (Panx1) Control/blocking peptide

AB-23246-P 100ug
EUR 164

Mouse pannexin 1 (Panx1) Control/blocking peptide

AB-23247-P 100ug
EUR 164

Mouse pannexin 2 (Panx2) Control/blocking peptide

AB-23260-P 100ug
EUR 164

Mouse aquaporin 2 (AQP2) Control/blocking peptide

AB-23265-P 100ug
EUR 164

Mouse uroplakin 3A (UPK3A) Control/blocking peptide

AB-23012-P 100ug
EUR 164

Mouse pannexin 1 (Panx1) Control/blocking peptide

AB-23016-P 100ug
EUR 164

Mouse mitoferrin-2 (Mfrn2) Control/blocking peptide

AB-23024-P 100ug
EUR 164

Mouse Mitoferrin-2 (Mfrn2) Control/blocking peptide

AB-23042-P 100ug
EUR 164

Mouse sideroflexin 4 (Sfxn4) Control/blocking peptide

AB-23060-P 100ug
EUR 164

Apoa5 (ab) Antibody

BF0615 200ul
EUR 376
Description: Apoa5 (ab) antibody detects endogenous levels of total Apoa5 (ab).

rHu PDGF-AB

AK8282-0002 2µg Ask for price

rHu PDGF-AB

AK8282-0010 10µg Ask for price

rHu PDGF-AB

AK8282-0100 100µg Ask for price

rHu PDGF-AB

AK8282-1000 1mg Ask for price

CAPA-IVM is an improved IVM system that includes a pre-maturation step (CAPA), followed by an IVM step, both in the presence of physiological compounds that promote oocyte developmental capacity.For DNA methylation analysis 20 CAPA-IVM blastocysts were compared to 12 COS blastocysts. For RNA-Seq analysis a separate set of 15 CAPA-IVM blastocysts were compared to 5 COS blastocysts.

COS embryos originated from 12 patients with PCOS (according to Rotterdam criteria) who underwent conventional ovarian stimulation.

For CAPA-IVM 23 women were treated for 3-5 days with highly purified hMG (HP-hMG) and no hCG trigger was given before oocyte retrieval. Oocytes were first cultured in pre-maturation medium (CAPA for 24 h containing C-type natriuretic peptide), followed by an IVM step (30 h) in medium containing FSH and Amphiregulin. After ICSI, Day 5 or 6 embryos in both groups were vitrified and used for post-bisulphite adaptor tagging (PBAT) DNA methylation analysis or RNA-seq gene expression analysis of individual embryos.

Data from specific genes and gDMRs were extracted from the PABT and RNA-seq datasets.CAPA-IVM blastocysts showed similar rates of methylation and gene expression at gDMRs compared to COS embryos. In addition, expression of major epigenetic regulators was similar between the groups.The embryos from the COS group were generated in a range of culture media. The CAPA-IVM embryos were all generated using the same sperm donor.