Does imprinted DNA methylation or imprinted gene expression differ between human blastocysts from conventional ovarian stimulation (COS) and an optimized two-step IVM method (CAPA-IVM) in age-matched polycystic ovary syndrome (PCOS) patients?No significant differences in imprinted DNA methylation and gene expression were detected between COS and CAPA-IVM blastocysts.
Animal models have revealed alterations in DNA methylation maintenance at imprinted germline differentially methylated regions (gDMRs) after use of ARTs. This effect increases as more ART interventions are applied to oocytes or embryos. IVM is a minimal-stimulation ART with reduced hormone-related side effects and risks for patients.
ELISA kit for Mouse ACV-AB (Activin AB) Kit |
E-EL-M2660 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 534 |
|
Description: A sandwich ELISA kit for quantitative measurement of Mouse ACV-AB (Activin AB) Kit in samples from Serum, Plasma, Cell supernatant |
Mouse Monoclonal Anti-Human Interleukin-2 IgG |
AB-10210 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Rat Monoclonal Anti-Mouse Interleukin-2 IgG |
AB-10510 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human Interleukin-3 IgG |
AB-10610 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human Interleukin-4 IgG |
AB-10710 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Rat Monoclonal Anti-Mouse Interleukin-4 IgG |
AB-10810 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human Interleukin-6 IgG |
AB-10910 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human Interleukin-7 IgG |
AB-11010 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human Interleukin-8 IgG |
AB-11110 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human Interleukin-10 IgG |
AB-11210 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Rat Monoclonal Anti-Mouse Interleukin-10 IgG |
AB-11310 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Rat Monoclonal Anti-Mouse Interleukin-12p40 IgG |
AB-11410 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human Interleukin-15 IgG |
AB-11510 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human CD8-biotinylated IgG |
AB-13410 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human CD3-biotinylated IgG |
AB-13810 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse monoclonal Anti-Human CD3 IgG, unlabled |
AB-14410 |
Alpha Diagnostics |
100 ug |
EUR 469 |
Mouse Monoclonal Anti-SARS Spike Protein IgG |
AB-15710 |
Alpha Diagnostics |
20 ug |
EUR 408 |
Mouse Monoclonal Anti-Human CD4-biotinylated IgG |
AB-16710 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-SARS Nucleocapsid protein IgG |
AB-17810 |
Alpha Diagnostics |
50 ug |
EUR 469 |
Mouse Monoclonal Anti-SARS Nucleocapsid Protein IgG |
AB-18010 |
Alpha Diagnostics |
50 ug |
EUR 469 |
Mouse Monoclonal Anti-Myelin Oligodendrocyte Glycoprotein IgG |
AB-19910 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Rat Monoclonal Anti-Mouse Interleukin-12p75 IgG |
AB-20910 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Rat Monoclonal Anti-Mouse Interleukin-2 receptor IgG |
AB-10310 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human Interleukin-2 receptor IgG |
AB-10410 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human Eotaxin-1 (CCL11) IgG |
AB-12610 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human CD1A (T6, LEU6) IgG |
AB-20610 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Hepatitis C Virus (HCV) NS3 IgG |
AB-15610 |
Alpha Diagnostics |
20 ug |
EUR 408 |
Mouse Monoclonal Anti-Human FAS (CD95) Blocking antibody IgG |
AB-20410 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human FAS (CD95) Activating antibody IgG |
AB-20510 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Human CD2 (T11, LFA-2) IgG |
AB-20710 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Hepatitis B Surface Antigen A (HBsAg) IgG |
AB-16310 |
Alpha Diagnostics |
1 ml |
EUR 347 |
Mouse PDGF-AB ELISA Kit |
LF-EK50843 |
Abfrontier |
1×96T |
EUR 648 |
Mouse BB-AB ELISA Kit |
EMB0647 |
Abclonal |
96Tests |
EUR 521 |
Mouse BP180-Ab ELISA Kit |
EMB0691 |
Abclonal |
96Tests |
EUR 521 |
Mouse CaM-ab ELISA Kit |
EMC0688 |
Abclonal |
96Tests |
EUR 521 |
Mouse CYT Ab ELISA Kit |
EMC0878 |
Abclonal |
96Tests |
EUR 521 |
Mouse DNP-AB ELISA Kit |
EMD0256 |
Abclonal |
96Tests |
EUR 521 |
Mouse ENA AB ELISA Kit |
EME0159 |
Abclonal |
96Tests |
EUR 521 |
Mouse ES-Ab ELISA Kit |
EME0181 |
Abclonal |
96Tests |
EUR 521 |
Mouse GAD-Ab ELISA Kit |
EMG0305 |
Abclonal |
96Tests |
EUR 521 |
Mouse GnRHR-Ab ELISA Kit |
EMG0347 |
Abclonal |
96Tests |
EUR 521 |
Mouse IFAa-AB ELISA Kit |
EMI0346 |
Abclonal |
96Tests |
EUR 521 |
Mouse MAG Ab ELISA Kit |
EMM0224 |
Abclonal |
96Tests |
EUR 521 |
Mouse MBP-Ab ELISA Kit |
EMM0248 |
Abclonal |
96Tests |
EUR 521 |
Mouse MC Ab ELISA Kit |
EMM0249 |
Abclonal |
96Tests |
EUR 521 |
Mouse MP-Ab ELISA Kit |
EMM0341 |
Abclonal |
96Tests |
EUR 521 |
Mouse NB-Ab ELISA Kit |
EMN0081 |
Abclonal |
96Tests |
EUR 521 |
Mouse RNP-AB ELISA Kit |
EMR0365 |
Abclonal |
96Tests |
EUR 521 |
Mouse TPO-AB ELISA Kit |
EMT0538 |
Abclonal |
96Tests |
EUR 521 |
Mouse UU-Ab ELISA Kit |
EMU0025 |
Abclonal |
96Tests |
EUR 521 |
Mouse Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit |
CN-02423M1 |
ChemNorm |
96T |
EUR 457 |
Mouse Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit |
CN-02423M2 |
ChemNorm |
48T |
EUR 306 |
Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit |
GA-E0148MS-48T |
GenAsia Biotech |
48T |
EUR 336 |
Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit |
GA-E0148MS-96T |
GenAsia Biotech |
96T |
EUR 534 |
Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit |
QY-E21208 |
Qayee Biotechnology |
96T |
EUR 361 |
Mouse Monoclonal Anti-Dengue Virus Type 2 envelop IgG, aff pure |
AB-14310 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-Hepatitis B Virus (HBsAg;AD & AY Antigens) IgG |
AB-15010 |
Alpha Diagnostics |
100 ug |
EUR 347 |
Mouse Monoclonal Anti-HIV-1 gp120 (PND, principal neutralizing domain) IgG |
AB-15110 |
Alpha Diagnostics |
100 ug |
EUR 347 |
ELISA kit for Mouse PDGF-AB (Platelet Derived Growth Factor AB) |
E-EL-M0631 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 534 |
|
Description: A sandwich ELISA kit for quantitative measurement of Mouse PDGF-AB (Platelet Derived Growth Factor AB) in samples from Serum, Plasma, Cell supernatant |
Mouse Activin AB (ACVAB) ELISA Kit |
CEA158Mu-10x96wellstestplate |
Cloud-Clone |
10x96-wells test plate |
EUR 4862.4 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Mouse Activin AB (ACVAB) ELISA Kit |
CEA158Mu-1x48wellstestplate |
Cloud-Clone |
1x48-wells test plate |
EUR 488.08 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Mouse Activin AB (ACVAB) ELISA Kit |
CEA158Mu-1x96wellstestplate |
Cloud-Clone |
1x96-wells test plate |
EUR 654.4 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Mouse Activin AB (ACVAB) ELISA Kit |
CEA158Mu-5x96wellstestplate |
Cloud-Clone |
5x96-wells test plate |
EUR 2644.8 |
|
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Mouse Activin AB (ACVAB) ELISA Kit |
4-CEA158Mu |
Cloud-Clone |
-
EUR 4913.00
-
EUR 2595.00
-
EUR 655.00
|
-
10 plates of 96 wells
-
5 plates of 96 wells
-
1 plate of 96 wells
|
|
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Mouse Activin AB (ACVAB) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Mouse Anti-Q-Ab ELISA Kit |
EMA0827 |
Abclonal |
96Tests |
EUR 521 |
Mouse BM-CSF Ab ELISA Kit |
EMB0673 |
Abclonal |
96Tests |
EUR 521 |
Mouse HSV I Ab ELISA Kit |
EMH0316 |
Abclonal |
96Tests |
EUR 521 |
Mouse IFN-α-Ab ELISA Kit |
EMI0048 |
Abclonal |
96Tests |
EUR 521 |
Mouse γ IFN-Ab ELISA Kit |
EMI0421 |
Abclonal |
96Tests |
EUR 521 |
Mouse LP Ab-lgA ELISA Kit |
EML0259 |
Abclonal |
96Tests |
EUR 521 |
Mouse LP Ab-lgG ELISA Kit |
EML0260 |
Abclonal |
96Tests |
EUR 521 |
Mouse LP Ab-lgM ELISA Kit |
EML0261 |
Abclonal |
96Tests |
EUR 521 |
Mouse LTF-AB-lgG ELISA Kit |
EML0302 |
Abclonal |
96Tests |
EUR 521 |
Mouse N-DNA-AB ELISA Kit |
EMN0082 |
Abclonal |
96Tests |
EUR 521 |
Mouse PR3 ab-lgG ELISA Kit |
EMP0717 |
Abclonal |
96Tests |
EUR 521 |
Mouse Scl-70 Ab ELISA Kit |
EMS0249 |
Abclonal |
96Tests |
EUR 521 |
Mouse Activin AB (ACVAB) CLIA Kit |
20-abx490029 |
Abbexa |
-
EUR 7973.00
-
EUR 4246.00
-
EUR 981.00
|
-
10 × 96 tests
-
5 × 96 tests
-
96 tests
|
|
Mouse Activin AB (ACVAB) ELISA Kit |
abx254671-96tests |
Abbexa |
96 tests |
EUR 754 |
|
PDGF-AB ELISA Kit (Mouse) (OKBB00241) |
OKBB00241 |
Aviva Systems Biology |
96 Tests |
EUR 544 |
Description: Description of target: The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 3 pg/mL |
ELISA kit for Mouse PDGF-AB |
EK5212 |
SAB |
96 tests |
EUR 519 |
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse PDGF-AB in samples from serum, plasma, tissue homogenates and other biological fluids. |
Mouse Meckelin (Mks3) Control/blocking peptide |
AB-23268-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse dermatopontin (Dpt) Control/blocking peptide |
AB-23026-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse pannexin 3 (Panx3) Control/blocking peptide |
AB-23242-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse pannexin 3 (Panx3) Control/blocking peptide |
AB-23243-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse pannexin 3 (Panx3) Control/blocking peptide |
AB-23244-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse pannexin 1 (Panx1) Control/blocking peptide |
AB-23246-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse pannexin 1 (Panx1) Control/blocking peptide |
AB-23247-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse pannexin 2 (Panx2) Control/blocking peptide |
AB-23260-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse aquaporin 2 (AQP2) Control/blocking peptide |
AB-23265-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse uroplakin 3A (UPK3A) Control/blocking peptide |
AB-23012-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse pannexin 1 (Panx1) Control/blocking peptide |
AB-23016-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse mitoferrin-2 (Mfrn2) Control/blocking peptide |
AB-23024-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse Mitoferrin-2 (Mfrn2) Control/blocking peptide |
AB-23042-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Mouse sideroflexin 4 (Sfxn4) Control/blocking peptide |
AB-23060-P |
Alpha Diagnostics |
100ug |
EUR 164 |
Apoa5 (ab) Antibody |
BF0615 |
Affbiotech |
200ul |
EUR 376 |
Description: Apoa5 (ab) antibody detects endogenous levels of total Apoa5 (ab). |
CAPA-IVM is an improved IVM system that includes a pre-maturation step (CAPA), followed by an IVM step, both in the presence of physiological compounds that promote oocyte developmental capacity.For DNA methylation analysis 20 CAPA-IVM blastocysts were compared to 12 COS blastocysts. For RNA-Seq analysis a separate set of 15 CAPA-IVM blastocysts were compared to 5 COS blastocysts.
COS embryos originated from 12 patients with PCOS (according to Rotterdam criteria) who underwent conventional ovarian stimulation.
For CAPA-IVM 23 women were treated for 3-5 days with highly purified hMG (HP-hMG) and no hCG trigger was given before oocyte retrieval. Oocytes were first cultured in pre-maturation medium (CAPA for 24 h containing C-type natriuretic peptide), followed by an IVM step (30 h) in medium containing FSH and Amphiregulin. After ICSI, Day 5 or 6 embryos in both groups were vitrified and used for post-bisulphite adaptor tagging (PBAT) DNA methylation analysis or RNA-seq gene expression analysis of individual embryos.
Data from specific genes and gDMRs were extracted from the PABT and RNA-seq datasets.CAPA-IVM blastocysts showed similar rates of methylation and gene expression at gDMRs compared to COS embryos. In addition, expression of major epigenetic regulators was similar between the groups.The embryos from the COS group were generated in a range of culture media. The CAPA-IVM embryos were all generated using the same sperm donor.