DNA methylation and mRNA expression of imprinted genes in blastocysts derived from an improved in vitro maturation method for oocytes from small antral follicles in polycystic ovary syndrome patients.

Does imprinted DNA methylation or imprinted gene expression differ between human blastocysts from conventional ovarian stimulation (COS) and an optimized two-step IVM method (CAPA-IVM) in age-matched polycystic ovary syndrome (PCOS) patients?No significant differences in imprinted DNA methylation and gene expression were detected between COS and CAPA-IVM blastocysts.

Animal models have revealed alterations in DNA methylation maintenance at imprinted germline differentially methylated regions (gDMRs) after use of ARTs. This effect increases as more ART interventions are applied to oocytes or embryos. IVM is a minimal-stimulation ART with reduced hormone-related side effects and risks for patients.

Rat Monoclonal Anti-Mouse CD11a IgG
AB-13510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD4 IgG
AB-14510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD8 IgG
AB-14810 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD4 IgG
AB-16510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD5 IgG
AB-17110 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD3 IgG
AB-17510 100 ug
EUR 347

Mouse Monoclonal Anti-SARS Spike IgG
AB-17910 50 ug
EUR 469

Mouse monoclonal Anti-HA-tag peptide IgG
AB-10110 100 ug
EUR 469

Mouse Monoclonal Anti-Human Interleukin-2 IgG
AB-10210 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-2 IgG
AB-10510 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-3 IgG
AB-10610 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-4 IgG
AB-10710 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-4 IgG
AB-10810 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-6 IgG
AB-10910 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-7 IgG
AB-11010 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-8 IgG
AB-11110 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-10 IgG
AB-11210 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-10 IgG
AB-11310 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-12p40 IgG
AB-11410 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-15 IgG
AB-11510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD8-FITC IgG
AB-13110 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD4-FITC IgG
AB-13210 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD8-biotinylated IgG
AB-13410 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD11b-FITC IgG
AB-13610 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD3-FITC IgG
AB-13710 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD3-biotinylated IgG
AB-13810 100 ug
EUR 347

Rat Monoclonal Anti-Mouse B220 (CD45R) IgG
AB-13910 100 ug
EUR 347

Mouse monoclonal Anti-Human CD3 IgG, unlabled
AB-14410 100 ug
EUR 469

Mouse Monoclonal Anti- HIV-1 p24 IgG
AB-15210 100 ug
EUR 347

Mouse Monoclonal Anti-HIV-2 gp39 IgG
AB-15310 100 ug
EUR 347

Mouse Monoclonal Anti-SARS Spike Protein IgG
AB-15710 20 ug
EUR 408

Mouse Monoclonal Anti-HIV-1 gp41 IgG
AB-15910 100 ug
EUR 347

Mouse Monoclonal Anti-HIV-2 gp39 IgG
AB-16110 1 ml
EUR 347

Mouse monoclonal Anti-HIV-2 p17 IgG
AB-16120 100 ug
EUR 469

Rat Monoclonal Anti-Mouse IFN-gamma IgG
AB-16610 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD4-biotinylated IgG
AB-16710 100 ug
EUR 347

Mouse Monoclonal Anti-SARS Nucleocapsid protein IgG
AB-17810 50 ug
EUR 469

Mouse Monoclonal Anti-SARS Nucleocapsid Protein IgG
AB-18010 50 ug
EUR 469

Mouse Monoclonal Anti-Myelin Oligodendrocyte Glycoprotein IgG
AB-19910 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-12p75 IgG
AB-20910 100 ug
EUR 347

Mouse Monoclonal Anti-Human c-Myc IgG
AB-21110 100 ug
EUR 347

Rat Monoclonal Anti-Mouse Interleukin-2 receptor IgG
AB-10310 100 ug
EUR 347

Mouse Monoclonal Anti-Human Interleukin-2 receptor IgG
AB-10410 100 ug
EUR 347

Hamster Mono Anti-Mouse CTLA-4 (CD152) IgG
AB-11610 100 ug
EUR 347

Mouse Monoclonal Anti-Human Eotaxin-1 (CCL11) IgG
AB-12610 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD90 Thy-1.1 IgG
AB-14010 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD90 Thy-1.2 IgG
AB-14110 100 ug
EUR 347

Rat Monoclonal Anti-Mouse CD90 Thy-1 IgG
AB-14210 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD1A (T6, LEU6) IgG
AB-20610 100 ug
EUR 347

ELISA kit for Mouse ACV-AB (Activin AB)  Kit
E-EL-M2660 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse ACV-AB (Activin AB)  Kit in samples from Serum, Plasma, Cell supernatant

ACV-AB ELISA Kit| Mouse Activin AB ELISA Kit
EF012990 96 Tests
EUR 689

Mouse Monoclonal Anti-Hepatitis C Virus (HCV) NS3 IgG
AB-15610 20 ug
EUR 408

Mouse Monoclonal Anti-Human FAS (CD95) Blocking antibody IgG
AB-20410 100 ug
EUR 347

Mouse Monoclonal Anti-Human FAS (CD95) Activating antibody IgG
AB-20510 100 ug
EUR 347

Mouse Monoclonal Anti-Human CD2 (T11, LFA-2) IgG
AB-20710 100 ug
EUR 347

Mouse Monoclonal Anti-Hepatitis B Surface Antigen A (HBsAg) IgG
AB-16310 1 ml
EUR 347

Mouse Monoclonal Anti-Dengue Virus Type 2 envelop IgG, aff pure
AB-14310 100 ug
EUR 347

Mouse Monoclonal Anti-Hepatitis B Virus (HBsAg;AD & AY Antigens) IgG
AB-15010 100 ug
EUR 347

Mouse Monoclonal Anti-HIV-1 gp120 (PND, principal neutralizing domain) IgG
AB-15110 100 ug
EUR 347

Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit
GA-E0148MS-48T 48T
EUR 336

Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit
GA-E0148MS-96T 96T
EUR 534

Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit
QY-E21208 96T
EUR 361

Mouse Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit
CN-02423M1 96T
EUR 457

Mouse Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit
CN-02423M2 48T
EUR 306

Mouse BB-AB ELISA Kit
EMB0647 96Tests
EUR 521

Mouse BP180-Ab ELISA Kit
EMB0691 96Tests
EUR 521

Mouse CaM-ab ELISA Kit
EMC0688 96Tests
EUR 521

Mouse CYT Ab ELISA Kit
EMC0878 96Tests
EUR 521

Mouse DNP-AB ELISA Kit
EMD0256 96Tests
EUR 521

Mouse ENA AB ELISA Kit
EME0159 96Tests
EUR 521

Mouse ES-Ab ELISA Kit
EME0181 96Tests
EUR 521

Mouse GAD-Ab ELISA Kit
EMG0305 96Tests
EUR 521

Mouse GnRHR-Ab ELISA Kit
EMG0347 96Tests
EUR 521

Mouse IFAa-AB ELISA Kit
EMI0346 96Tests
EUR 521

Mouse MAG Ab ELISA Kit
EMM0224 96Tests
EUR 521

Mouse MBP-Ab ELISA Kit
EMM0248 96Tests
EUR 521

Mouse MC Ab ELISA Kit
EMM0249 96Tests
EUR 521

Mouse MP-Ab ELISA Kit
EMM0341 96Tests
EUR 521

Mouse NB-Ab ELISA Kit
EMN0081 96Tests
EUR 521

Mouse RNP-AB ELISA Kit
EMR0365 96Tests
EUR 521

Mouse TPO-AB ELISA Kit
EMT0538 96Tests
EUR 521

Mouse UU-Ab ELISA Kit
EMU0025 96Tests
EUR 521

Mouse Thyroid Ab ELISA Kit
ELA-E0844m 96 Tests
EUR 865

Mouse PDGF-AB ELISA Kit
LF-EK50843 1×96T
EUR 648

AB-MECA
HY-19365 5mg
EUR 380

AB-423
HY-112142 100mg
EUR 2058

AB-680
HY-125286 10mg
EUR 1772

Soyasaponin Ab
HY-N3026 5mg
EUR 282

Activin AB
PR15011 5 ug
EUR 481

ELISA kit for Mouse PDGF-AB (Platelet Derived Growth Factor AB)
E-EL-M0631 1 plate of 96 wells
EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse PDGF-AB (Platelet Derived Growth Factor AB) in samples from Serum, Plasma, Cell supernatant

Mouse Activin AB (ACVAB) ELISA Kit
CEA158Mu-10x96wellstestplate 10x96-wells test plate
EUR 4862.4
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit
CEA158Mu-1x48wellstestplate 1x48-wells test plate
EUR 488.08
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit
CEA158Mu-1x96wellstestplate 1x96-wells test plate
EUR 654.4
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit
CEA158Mu-5x96wellstestplate 5x96-wells test plate
EUR 2644.8
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit
4-CEA158Mu
  • EUR 4913.00
  • EUR 2595.00
  • EUR 655.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Mouse Activin AB (ACVAB) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Anti-Q-Ab ELISA Kit
EMA0827 96Tests
EUR 521

Mouse BM-CSF Ab ELISA Kit
EMB0673 96Tests
EUR 521

Mouse HSV I Ab ELISA Kit
EMH0316 96Tests
EUR 521

Mouse IFN-α-Ab ELISA Kit
EMI0048 96Tests
EUR 521

Mouse γ IFN-Ab ELISA Kit
EMI0421 96Tests
EUR 521

Mouse LP Ab-lgA ELISA Kit
EML0259 96Tests
EUR 521

Mouse LP Ab-lgG ELISA Kit
EML0260 96Tests
EUR 521

Mouse LP Ab-lgM ELISA Kit
EML0261 96Tests
EUR 521

Mouse LTF-AB-lgG ELISA Kit
EML0302 96Tests
EUR 521

Mouse N-DNA-AB ELISA Kit
EMN0082 96Tests
EUR 521

Mouse PR3 ab-lgG ELISA Kit
EMP0717 96Tests
EUR 521

Mouse Scl-70 Ab ELISA Kit
EMS0249 96Tests
EUR 521

Mouse Activin AB (ACVAB) ELISA Kit
abx254671-96tests 96 tests
EUR 754

Mouse Activin AB (ACVAB) CLIA Kit
20-abx490029
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

PDGF-AB ELISA Kit (Mouse) (OKBB00241)
OKBB00241 96 Tests
EUR 544
Description: Description of target: The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 3 pg/mL

ELISA kit for Mouse PDGF-AB
EK5212 96 tests
EUR 519
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse PDGF-AB in samples from serum, plasma, tissue homogenates and other biological fluids.

PDGF-AB/ Rat PDGF- AB ELISA Kit
ELA-E0112r 96 Tests
EUR 886

MBP-Ab/ Rat MBP- Ab ELISA Kit
ELA-E0170r 96 Tests
EUR 886

MC Ab/ Rat MC Ab ELISA Kit
ELA-E0390r 96 Tests
EUR 886

TPO-Ab/ Rat TPO- Ab ELISA Kit
ELA-E0442r 96 Tests
EUR 886

Thyroid Ab/ Rat Thyroid Ab ELISA Kit
ELA-E0844r 96 Tests
EUR 886

GNRHR-Ab/ Rat GNRHR- Ab ELISA Kit
ELA-E0962r 96 Tests
EUR 886

MAG Ab/ Rat MAG Ab ELISA Kit
ELA-E0995r 96 Tests
EUR 886

DNP-Ab/ Rat DNP- Ab ELISA Kit
ELA-E1122r 96 Tests
EUR 886

ENA-Ab/ Rat ENA- Ab ELISA Kit
ELA-E1124r 96 Tests
EUR 886

RNP-Ab/ Rat RNP- Ab ELISA Kit
ELA-E1129r 96 Tests
EUR 886

Mouse dermatopontin (Dpt) Control/blocking peptide
AB-23026-P 100ug
EUR 164

Mouse Meckelin (Mks3) Control/blocking peptide
AB-23268-P 100ug
EUR 164

Mouse uroplakin 3A (UPK3A) Control/blocking peptide
AB-23012-P 100ug
EUR 164

Mouse pannexin 1 (Panx1) Control/blocking peptide
AB-23016-P 100ug
EUR 164

Mouse mitoferrin-2 (Mfrn2) Control/blocking peptide
AB-23024-P 100ug
EUR 164

Mouse Mitoferrin-2 (Mfrn2) Control/blocking peptide
AB-23042-P 100ug
EUR 164

Mouse sideroflexin 4 (Sfxn4) Control/blocking peptide
AB-23060-P 100ug
EUR 164

Mouse pannexin 3 (Panx3) Control/blocking peptide
AB-23242-P 100ug
EUR 164

Mouse pannexin 3 (Panx3) Control/blocking peptide
AB-23243-P 100ug
EUR 164

Mouse pannexin 3 (Panx3) Control/blocking peptide
AB-23244-P 100ug
EUR 164

Mouse pannexin 1 (Panx1) Control/blocking peptide
AB-23246-P 100ug
EUR 164

Mouse pannexin 1 (Panx1) Control/blocking peptide
AB-23247-P 100ug
EUR 164

Mouse pannexin 2 (Panx2) Control/blocking peptide
AB-23260-P 100ug
EUR 164

Mouse aquaporin 2 (AQP2) Control/blocking peptide
AB-23265-P 100ug
EUR 164

Ab Rabbit GluR2*
P40014-100uL 100uL Ask for price

Ab Rabbit MARCKS*
P40017-100uL 100uL Ask for price

Ab MS GluR1*
P80000-100uL 100uL Ask for price

Activin AB, CF
PR15011CF 5 ug
EUR 481

PDGF - AB Human
PR27062 2 ug
EUR 191

CAPA-IVM is an improved IVM system that includes a pre-maturation step (CAPA), followed by an IVM step, both in the presence of physiological compounds that promote oocyte developmental capacity.For DNA methylation analysis 20 CAPA-IVM blastocysts were compared to 12 COS blastocysts. For RNA-Seq analysis a separate set of 15 CAPA-IVM blastocysts were compared to 5 COS blastocysts.

COS embryos originated from 12 patients with PCOS (according to Rotterdam criteria) who underwent conventional ovarian stimulation.

For CAPA-IVM 23 women were treated for 3-5 days with highly purified hMG (HP-hMG) and no hCG trigger was given before oocyte retrieval. Oocytes were first cultured in pre-maturation medium (CAPA for 24 h containing C-type natriuretic peptide), followed by an IVM step (30 h) in medium containing FSH and Amphiregulin. After ICSI, Day 5 or 6 embryos in both groups were vitrified and used for post-bisulphite adaptor tagging (PBAT) DNA methylation analysis or RNA-seq gene expression analysis of individual embryos.

Data from specific genes and gDMRs were extracted from the PABT and RNA-seq datasets.CAPA-IVM blastocysts showed similar rates of methylation and gene expression at gDMRs compared to COS embryos. In addition, expression of major epigenetic regulators was similar between the groups.The embryos from the COS group were generated in a range of culture media. The CAPA-IVM embryos were all generated using the same sperm donor.

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