Bioslunecnice

Does imprinted DNA methylation or imprinted gene expression differ between human blastocysts from conventional ovarian stimulation (COS) and an optimized two-step IVM method (CAPA-IVM) in age-matched polycystic ovary syndrome (PCOS) patients?No significant differences in imprinted DNA methylation and gene expression were detected between COS and CAPA-IVM blastocysts.

Animal models have revealed alterations in DNA methylation maintenance at imprinted germline differentially methylated regions (gDMRs) after use of ARTs. This effect increases as more ART interventions are applied to oocytes or embryos. IVM is a minimal-stimulation ART with reduced hormone-related side effects and risks for patients.

Rat Monoclonal Anti-Mouse CD11a IgG
AB-13510	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human CD4 IgG
AB-14510	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human CD8 IgG
AB-14810	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse CD4 IgG
AB-16510	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human CD5 IgG
AB-17110	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse CD3 IgG
AB-17510	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-SARS Spike IgG
AB-17910	Alpha Diagnostics	50 ug	EUR 469

ACV-AB ELISA Kit| Mouse Activin AB ELISA Kit
EF012990	Lifescience Market	96 Tests	EUR 689

ELISA kit for Mouse ACV-AB (Activin AB)  Kit
E-EL-M2660	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse ACV-AB (Activin AB)  Kit in samples from Serum, Plasma, Cell supernatant

Mouse monoclonal Anti-HA-tag peptide IgG
AB-10110	Alpha Diagnostics	100 ug	EUR 469

Mouse Monoclonal Anti-Human Interleukin-2 IgG
AB-10210	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse Interleukin-2 IgG
AB-10510	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human Interleukin-3 IgG
AB-10610	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human Interleukin-4 IgG
AB-10710	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse Interleukin-4 IgG
AB-10810	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human Interleukin-6 IgG
AB-10910	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human Interleukin-7 IgG
AB-11010	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human Interleukin-8 IgG
AB-11110	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human Interleukin-10 IgG
AB-11210	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse Interleukin-10 IgG
AB-11310	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse Interleukin-12p40 IgG
AB-11410	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human Interleukin-15 IgG
AB-11510	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human CD8-FITC IgG
AB-13110	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human CD4-FITC IgG
AB-13210	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human CD8-biotinylated IgG
AB-13410	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse CD11b-FITC IgG
AB-13610	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human CD3-FITC IgG
AB-13710	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human CD3-biotinylated IgG
AB-13810	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse B220 (CD45R) IgG
AB-13910	Alpha Diagnostics	100 ug	EUR 347

Mouse monoclonal Anti-Human CD3 IgG, unlabled
AB-14410	Alpha Diagnostics	100 ug	EUR 469

Mouse Monoclonal Anti- HIV-1 p24 IgG
AB-15210	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-HIV-2 gp39 IgG
AB-15310	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-SARS Spike Protein IgG
AB-15710	Alpha Diagnostics	20 ug	EUR 408

Mouse Monoclonal Anti-HIV-1 gp41 IgG
AB-15910	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-HIV-2 gp39 IgG
AB-16110	Alpha Diagnostics	1 ml	EUR 347

Mouse monoclonal Anti-HIV-2 p17 IgG
AB-16120	Alpha Diagnostics	100 ug	EUR 469

Rat Monoclonal Anti-Mouse IFN-gamma IgG
AB-16610	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human CD4-biotinylated IgG
AB-16710	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-SARS Nucleocapsid protein IgG
AB-17810	Alpha Diagnostics	50 ug	EUR 469

Mouse Monoclonal Anti-SARS Nucleocapsid Protein IgG
AB-18010	Alpha Diagnostics	50 ug	EUR 469

Mouse Monoclonal Anti-Myelin Oligodendrocyte Glycoprotein IgG
AB-19910	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse Interleukin-12p75 IgG
AB-20910	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human c-Myc IgG
AB-21110	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse Interleukin-2 receptor IgG
AB-10310	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human Interleukin-2 receptor IgG
AB-10410	Alpha Diagnostics	100 ug	EUR 347

Hamster Mono Anti-Mouse CTLA-4 (CD152) IgG
AB-11610	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human Eotaxin-1 (CCL11) IgG
AB-12610	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse CD90 Thy-1.1 IgG
AB-14010	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse CD90 Thy-1.2 IgG
AB-14110	Alpha Diagnostics	100 ug	EUR 347

Rat Monoclonal Anti-Mouse CD90 Thy-1 IgG
AB-14210	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human CD1A (T6, LEU6) IgG
AB-20610	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Hepatitis C Virus (HCV) NS3 IgG
AB-15610	Alpha Diagnostics	20 ug	EUR 408

Mouse Monoclonal Anti-Human FAS (CD95) Blocking antibody IgG
AB-20410	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human FAS (CD95) Activating antibody IgG
AB-20510	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Human CD2 (T11, LFA-2) IgG
AB-20710	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Hepatitis B Surface Antigen A (HBsAg) IgG
AB-16310	Alpha Diagnostics	1 ml	EUR 347

Mouse Thyroid Ab ELISA Kit
ELA-E0844m	Lifescience Market	96 Tests	EUR 865

Mouse PDGF-AB ELISA Kit
LF-EK50843	Abfrontier	1×96T	EUR 648

Mouse BB-AB ELISA Kit
EMB0647	Abclonal	96Tests	EUR 521

Mouse BP180-Ab ELISA Kit
EMB0691	Abclonal	96Tests	EUR 521

Mouse CaM-ab ELISA Kit
EMC0688	Abclonal	96Tests	EUR 521

Mouse CYT Ab ELISA Kit
EMC0878	Abclonal	96Tests	EUR 521

Mouse DNP-AB ELISA Kit
EMD0256	Abclonal	96Tests	EUR 521

Mouse ENA AB ELISA Kit
EME0159	Abclonal	96Tests	EUR 521

Mouse ES-Ab ELISA Kit
EME0181	Abclonal	96Tests	EUR 521

Mouse GAD-Ab ELISA Kit
EMG0305	Abclonal	96Tests	EUR 521

Mouse GnRHR-Ab ELISA Kit
EMG0347	Abclonal	96Tests	EUR 521

Mouse IFAa-AB ELISA Kit
EMI0346	Abclonal	96Tests	EUR 521

Mouse MAG Ab ELISA Kit
EMM0224	Abclonal	96Tests	EUR 521

Mouse MBP-Ab ELISA Kit
EMM0248	Abclonal	96Tests	EUR 521

Mouse MC Ab ELISA Kit
EMM0249	Abclonal	96Tests	EUR 521

Mouse MP-Ab ELISA Kit
EMM0341	Abclonal	96Tests	EUR 521

Mouse NB-Ab ELISA Kit
EMN0081	Abclonal	96Tests	EUR 521

Mouse RNP-AB ELISA Kit
EMR0365	Abclonal	96Tests	EUR 521

Mouse TPO-AB ELISA Kit
EMT0538	Abclonal	96Tests	EUR 521

Mouse UU-Ab ELISA Kit
EMU0025	Abclonal	96Tests	EUR 521

Mouse Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit
CN-02423M1	ChemNorm	96T	EUR 457

Mouse Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit
CN-02423M2	ChemNorm	48T	EUR 306

Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit
GA-E0148MS-48T	GenAsia Biotech	48T	EUR 336

Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit
GA-E0148MS-96T	GenAsia Biotech	96T	EUR 534

Mouse Platelet-Derived Growth Factor AB(PDGF-AB)ELISA Kit
QY-E21208	Qayee Biotechnology	96T	EUR 361

Mouse Monoclonal Anti-Dengue Virus Type 2 envelop IgG, aff pure
AB-14310	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-Hepatitis B Virus (HBsAg;AD & AY Antigens) IgG
AB-15010	Alpha Diagnostics	100 ug	EUR 347

Mouse Monoclonal Anti-HIV-1 gp120 (PND, principal neutralizing domain) IgG
AB-15110	Alpha Diagnostics	100 ug	EUR 347

ELISA kit for Mouse PDGF-AB (Platelet Derived Growth Factor AB)
E-EL-M0631	Elabscience Biotech	1 plate of 96 wells	EUR 534
Description: A sandwich ELISA kit for quantitative measurement of Mouse PDGF-AB (Platelet Derived Growth Factor AB) in samples from Serum, Plasma, Cell supernatant

Soyasaponin Ab
HY-N3026	MedChemExpress	5mg	EUR 282

AB-423
HY-112142	MedChemExpress	100mg	EUR 2058

AB-680
HY-125286	MedChemExpress	10mg	EUR 1772

AB-MECA
HY-19365	MedChemExpress	5mg	EUR 380

Activin AB
PR15011	Neuromics	5 ug	EUR 481

Mouse Activin AB (ACVAB) ELISA Kit
CEA158Mu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 4862.4
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit
CEA158Mu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 488.08
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit
CEA158Mu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 654.4
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit
CEA158Mu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2644.8
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse Activin AB (ACVAB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Mouse Activin AB (ACVAB) ELISA Kit
4-CEA158Mu	Cloud-Clone	EUR 4913.00 EUR 2595.00 EUR 655.00	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Mouse Activin AB (ACVAB) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Anti-Q-Ab ELISA Kit
EMA0827	Abclonal	96Tests	EUR 521

Mouse BM-CSF Ab ELISA Kit
EMB0673	Abclonal	96Tests	EUR 521

Mouse HSV I Ab ELISA Kit
EMH0316	Abclonal	96Tests	EUR 521

Mouse IFN-α-Ab ELISA Kit
EMI0048	Abclonal	96Tests	EUR 521

Mouse γ IFN-Ab ELISA Kit
EMI0421	Abclonal	96Tests	EUR 521

Mouse LP Ab-lgA ELISA Kit
EML0259	Abclonal	96Tests	EUR 521

Mouse LP Ab-lgG ELISA Kit
EML0260	Abclonal	96Tests	EUR 521

Mouse LP Ab-lgM ELISA Kit
EML0261	Abclonal	96Tests	EUR 521

Mouse LTF-AB-lgG ELISA Kit
EML0302	Abclonal	96Tests	EUR 521

Mouse N-DNA-AB ELISA Kit
EMN0082	Abclonal	96Tests	EUR 521

Mouse PR3 ab-lgG ELISA Kit
EMP0717	Abclonal	96Tests	EUR 521

Mouse Scl-70 Ab ELISA Kit
EMS0249	Abclonal	96Tests	EUR 521

Mouse Activin AB (ACVAB) CLIA Kit
20-abx490029	Abbexa	EUR 7973.00 EUR 4246.00 EUR 981.00	10 × 96 tests 5 × 96 tests 96 tests

Mouse Activin AB (ACVAB) ELISA Kit
abx254671-96tests	Abbexa	96 tests	EUR 754

PDGF-AB ELISA Kit (Mouse) (OKBB00241)
OKBB00241	Aviva Systems Biology	96 Tests	EUR 544
Description: Description of target: The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 3 pg/mL

ELISA kit for Mouse PDGF-AB
EK5212	SAB	96 tests	EUR 519
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse PDGF-AB in samples from serum, plasma, tissue homogenates and other biological fluids.

PDGF-AB/ Rat PDGF- AB ELISA Kit
ELA-E0112r	Lifescience Market	96 Tests	EUR 886

MBP-Ab/ Rat MBP- Ab ELISA Kit
ELA-E0170r	Lifescience Market	96 Tests	EUR 886

MC Ab/ Rat MC Ab ELISA Kit
ELA-E0390r	Lifescience Market	96 Tests	EUR 886

TPO-Ab/ Rat TPO- Ab ELISA Kit
ELA-E0442r	Lifescience Market	96 Tests	EUR 886

Thyroid Ab/ Rat Thyroid Ab ELISA Kit
ELA-E0844r	Lifescience Market	96 Tests	EUR 886

GNRHR-Ab/ Rat GNRHR- Ab ELISA Kit
ELA-E0962r	Lifescience Market	96 Tests	EUR 886

MAG Ab/ Rat MAG Ab ELISA Kit
ELA-E0995r	Lifescience Market	96 Tests	EUR 886

DNP-Ab/ Rat DNP- Ab ELISA Kit
ELA-E1122r	Lifescience Market	96 Tests	EUR 886

ENA-Ab/ Rat ENA- Ab ELISA Kit
ELA-E1124r	Lifescience Market	96 Tests	EUR 886

RNP-Ab/ Rat RNP- Ab ELISA Kit
ELA-E1129r	Lifescience Market	96 Tests	EUR 886

Mouse Meckelin (Mks3) Control/blocking peptide
AB-23268-P	Alpha Diagnostics	100ug	EUR 164

Mouse dermatopontin (Dpt) Control/blocking peptide
AB-23026-P	Alpha Diagnostics	100ug	EUR 164

Mouse pannexin 3 (Panx3) Control/blocking peptide
AB-23242-P	Alpha Diagnostics	100ug	EUR 164

Mouse pannexin 3 (Panx3) Control/blocking peptide
AB-23243-P	Alpha Diagnostics	100ug	EUR 164

Mouse pannexin 3 (Panx3) Control/blocking peptide
AB-23244-P	Alpha Diagnostics	100ug	EUR 164

Mouse pannexin 1 (Panx1) Control/blocking peptide
AB-23246-P	Alpha Diagnostics	100ug	EUR 164

Mouse pannexin 1 (Panx1) Control/blocking peptide
AB-23247-P	Alpha Diagnostics	100ug	EUR 164

Mouse pannexin 2 (Panx2) Control/blocking peptide
AB-23260-P	Alpha Diagnostics	100ug	EUR 164

Mouse aquaporin 2 (AQP2) Control/blocking peptide
AB-23265-P	Alpha Diagnostics	100ug	EUR 164

Mouse uroplakin 3A (UPK3A) Control/blocking peptide
AB-23012-P	Alpha Diagnostics	100ug	EUR 164

Mouse pannexin 1 (Panx1) Control/blocking peptide
AB-23016-P	Alpha Diagnostics	100ug	EUR 164

Mouse mitoferrin-2 (Mfrn2) Control/blocking peptide
AB-23024-P	Alpha Diagnostics	100ug	EUR 164

Mouse Mitoferrin-2 (Mfrn2) Control/blocking peptide
AB-23042-P	Alpha Diagnostics	100ug	EUR 164

Mouse sideroflexin 4 (Sfxn4) Control/blocking peptide
AB-23060-P	Alpha Diagnostics	100ug	EUR 164

Apoa5 (ab) Antibody
BF0615	Affbiotech	200ul	EUR 376
Description: Apoa5 (ab) antibody detects endogenous levels of total Apoa5 (ab).

rHu PDGF-AB
AK8282-0002	Akron Biotech	2µg	Ask for price

rHu PDGF-AB
AK8282-0010	Akron Biotech	10µg	Ask for price

rHu PDGF-AB
AK8282-0100	Akron Biotech	100µg	Ask for price

rHu PDGF-AB
AK8282-1000	Akron Biotech	1mg	Ask for price

CAPA-IVM is an improved IVM system that includes a pre-maturation step (CAPA), followed by an IVM step, both in the presence of physiological compounds that promote oocyte developmental capacity.For DNA methylation analysis 20 CAPA-IVM blastocysts were compared to 12 COS blastocysts. For RNA-Seq analysis a separate set of 15 CAPA-IVM blastocysts were compared to 5 COS blastocysts.

COS embryos originated from 12 patients with PCOS (according to Rotterdam criteria) who underwent conventional ovarian stimulation.

For CAPA-IVM 23 women were treated for 3-5 days with highly purified hMG (HP-hMG) and no hCG trigger was given before oocyte retrieval. Oocytes were first cultured in pre-maturation medium (CAPA for 24 h containing C-type natriuretic peptide), followed by an IVM step (30 h) in medium containing FSH and Amphiregulin. After ICSI, Day 5 or 6 embryos in both groups were vitrified and used for post-bisulphite adaptor tagging (PBAT) DNA methylation analysis or RNA-seq gene expression analysis of individual embryos.

Data from specific genes and gDMRs were extracted from the PABT and RNA-seq datasets.CAPA-IVM blastocysts showed similar rates of methylation and gene expression at gDMRs compared to COS embryos. In addition, expression of major epigenetic regulators was similar between the groups.The embryos from the COS group were generated in a range of culture media. The CAPA-IVM embryos were all generated using the same sperm donor.