If the nucleotide sequences at the ends of a particular region of DNA are known, the intervening fragment can be amplified directly by polymerase chain reaction (PCR). Here we describe the basic technique of PCR and three situations in which it is used. PCR relies on the ability to alternately denature (melt) double-stranded DNA molecules and anneal (anneal) complementary single strands in a controlled manner.
As in the membrane hybridization assay described above, the presence of non-complementary strands in a mixture has little effect on the base-pairing of complementary single DNA strands or complementary regions of strands. The second requirement for PCR-JA-Elisa-sarjat is the ability to synthesize oligonucleotides at least 18 to 20 nucleotides long with a defined sequence. Such synthetic nucleotides can be easily produced with automated instruments based on the standard reaction scheme.
A typical PCR procedure begins with the heat denaturation of a single-stranded DNA sample. Next, two synthetic oligonucleotides complementary to the 3′ ends of the DNA segment of interest are added in large excess to the denatured DNA and the temperature is lowered to 50–60 C. These specific oligonucleotides, found in very high concentrations, will hybridize to their complementary sequences in the DNA sample, while the long strands in the DNA sample will remain separate due to their low concentration.
The annealed oligonucleotides then serve as primers for DNA strand synthesis in the presence of deoxynucleotides (dNTPs) and a temperature-resistant DNA polymerase, such as that from Thermus aquaticus (a bacterium that lives in hot springs). This enzyme, called Taq polymerase, can remain active even after heating to 95 C and can extend primers at temperatures up to 72 C. When the synthesis is complete, the entire mixture is heated to 95 C to melt the newly formed DNA duplexes formed.
After the temperature is lowered again, another cycle of synthesis is carried out because the excess primer is still present. Repeated cycles of fusion (heating) and synthesis (cooling) rapidly amplify the sequence of interest. In each cycle, the number of copies of the sequence between the primer sites doubles; thus, the desired sequence increases exponentially, about a million-fold after 20 cycles, while all other sequences in the original DNA sample remain unamplified.
Product name: Jasmonic Acid (JA), ELISA Kit
Full product name
Vegetable Jasmonic Acid (JA) ELISA Kit
Product gene name
JA ELISA kit
For research use only
For research use only. It should not be used in diagnostic procedures.
Species reactivity: Plant
Test type: Quantitative sandwich
Detection range: 0.25nmol/L-8nmol/L
Intra-assay precision: The intra-assay CV (%) is less than 15%.
The inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not by OD values.
Preparation and Storage
Store all reagents at 2-8 degrees C
Information selected from the online data sheet is extracted from bioinformatics databases, sometimes resulting in the ambiguous or non-relevant product information. It is the customer’s responsibility to review, verify and evaluate the information to ensure it matches their requirements prior to purchasing the kit. Our ELISA Kit assays are dynamic research tools and may be updated and improved from time to time.
If the format of this assay is important to you, please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current trial details. We cannot guarantee that the sample manual posted online is the most up-to-date manual, it is intended to serve as an example only. Consult the instructions for use provided with the assay kit for precise details.
On occasion, small volumes of JA Elisa kit vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly spin the vial in a tabletop centrifuge to dislodge any liquid in the vial cap. Certain products may require dry ice shipping and an additional dry ice fee may apply.
MBS9315634 is a ready-to-use microwell and strip plate ELISA (Enzyme-Linked Immunosorbent Assay) Kit for testing the presence of the target analytes of the Jasmonic Acid (JA) ELISA Kit in biological samples. The concentration gradients of the kit standards or positive controls generate a theoretical detection range of the kit in biological research samples containing JA.
The analytical biochemical ELISA technique of the MBS9315634 kit is based on JA antibody-JA antigen (immunosorbent) interactions and an HRP colourimetric detection system to detect JA antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant JA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays that evaluated reproducibility identified intra-assay CV (%) and inter-assay CV (%).
Product related information for kit ja ELISA
Background/Introduction: This quantitative sandwich ELISA kit is for laboratory research/reagent use only, not for drug, home, therapeutic, or testing applications! This kit is intended to be used to determine the level of JA (hereinafter referred to as „analyte“) in samples of undiluted original plant tissue homogenates. For other sample types, contact technical support to determine compatibility with this assay. This kit is not suitable for testing non-biological sources of substances.
All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic, or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe the intended use will not meet our acceptable guidelines.
While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource shall not be responsible for any omissions or errors contained in this document. MyBioSource reserves the right to make changes to this datasheet at any time without notice.